The new-look site is scheduled to go live in early November and will offer advertising formats currently unavailable, such as double skyscrapers.
The revamp marks the first time Condè Nast has invested in the site since its launch seven years ago.
The relaunch is not the only move Condè Nast is making to reinvigorate the Glamour brand. The publisher recently concluded a pan-European advertising review of the title, awarding the account to DDB Paris.
The advertising pitch also involved JWT London, BETC Euro RSCG and Ogilvy Advertising.
In its last campaign, Glamour used the slogan "Fits into your life and your handbag".
In the most recent magazine ABCs, for January to June 2008, Glamour boosted its position at the top of the women's magazine market and recorded a circulation of 551,351 - a period-on-period increase of 0.2%.
Glamour.com is not ABCe registered. However, according to a spokesman, it attracts eight million page impressions and 500,000 unique users per month.