The fresh funding comes as the micro-blogging site appoints two new directors to its board.
Twitter said the investment had come from the venture capital firm Kleiner Perkins Caulfield Byers and existing investors.
Twitter has now raised around $360m since its launch in 2007.
It has also appointed two new board members, Flipboard entrepreneur Mike McCue, and the boss of Google-owned digital ad technology company DoubleClick, David Rosenblatt.
The appointments follow a number of personnel changes at the company.
The latest departure was the company’s vice-president of product, Jason Goldman, who announced his resignation on stage at the Le Web conference in Paris.
Twitter, based in San Francisco, employs around 350 people.